DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.

We have developed a cell-free system for studying the synthesis of mRNA in mammalian cells. The system consists of a dialyzed and concentrated whole-cell extract derived from HeLa cells, small molecules and cofactors needed for transcription, and exogenously added DNA. Accurate transcription by RNA...

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Detalhes bibliográficos
Main Authors: Manley, J L, Fire, A, Cano, A, Sharp, P A, Gefter, M L
Formato: Text
Idioma:Inglês
Publicado em: 1980
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Acesso em linha:https://pmc.ncbi.nlm.nih.gov/articles/PMC349725/
https://pubmed.ncbi.nlm.nih.gov/6933441
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