Dam methylase from Escherichia coli: kinetic studies using modified DNA oligomers: hemimethylated substrates.
We have measured steady-state kinetics of the N6-adenine methyltransferase Dam Mtase using as substrates non-selfcomplementary tetradecamer duplexs (d[GCCGGATCTAGACG]-d[CGTCTAGATCC-GGC]) containing the hemimethylated GATC target sequence in one or the other strand and modifications in the GATC targe...
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Main Authors: | , , , , , |
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Format: | Text |
Language: | English |
Published: |
1995
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Online Access: | https://pmc.ncbi.nlm.nih.gov/articles/PMC307261/ https://pubmed.ncbi.nlm.nih.gov/7478992 |
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