Dam methylase from Escherichia coli: kinetic studies using modified DNA oligomers: hemimethylated substrates.

We have measured steady-state kinetics of the N6-adenine methyltransferase Dam Mtase using as substrates non-selfcomplementary tetradecamer duplexs (d[GCCGGATCTAGACG]-d[CGTCTAGATCC-GGC]) containing the hemimethylated GATC target sequence in one or the other strand and modifications in the GATC targe...

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Bibliographic Details
Main Authors: Marzabal, S, DuBois, S, Thielking, V, Cano, A, Eritja, R, Guschlbauer, W
Format: Text
Language:English
Published: 1995
Online Access:https://pmc.ncbi.nlm.nih.gov/articles/PMC307261/
https://pubmed.ncbi.nlm.nih.gov/7478992
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