Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity
Abstract Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination m...
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| Hlavní autoři: | , , , , , , , , , , , |
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| Médium: | Artigo |
| Jazyk: | angličtina |
| Vydáno: |
2023
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| On-line přístup: | https://doi.org/10.1038/s41587-023-01893-7 https://www.nature.com/articles/s41587-023-01893-7.pdf |
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