Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity

Abstract Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination m...

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Detaylı Bibliyografya
Asıl Yazarlar: Lucie Ludvíková, Emma Simon, Mathieu Deygas, Thomas Panier, Marie‐Aude Plamont, Jean Ollion, Alison G. Tebo, Matthieu Piel, Ludovic Jullien, Lydia Robert, Thomas Le Saux, Agathe Espagne
Materyal Türü: Artigo
Dil:İngilizce
Baskı/Yayın Bilgisi: 2023
Online Erişim:https://doi.org/10.1038/s41587-023-01893-7
https://www.nature.com/articles/s41587-023-01893-7.pdf
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