Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research and its industrialization. This study set out to establish standards-based solutions to improve the accuracy and reproducibility of metag...

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Autors principals: Dieter M. Tourlousse, Koji Narita, Takamasa Miura, Mitsuo Sakamoto, Akiko Ohashi, Keita Shiina, Masami Matsuda, Daisuke Miura, Mamiko Shimamura, Yoshifumi Ohyama, Atsushi Yamazoe, Y. Uchino, Keishi Kameyama, Shingo Arioka, Jiro Kataoka, Takayoshi Hisada, Kazuyuki Fujii, Shunsuke Takahashi, Miho Kuroiwa, Masatomo Rokushima, Mitsue Nishiyama, Yoshiki Tanaka, Takuya Fuchikami, Hitomi Aoki, Satoshi Kira, Ryo Koyanagi, Takeshi Naito, Morie Nishiwaki, Hirotaka Kumagai, Mikiko Konda, Ken Kasahara, Moriya Ohkuma, Hiroko Kawasaki, Yuji Sekiguchi, Jun Terauchi
Format: Artigo
Idioma:anglès
Publicat: 2021
Accés en línia:https://doi.org/10.1186/s40168-021-01048-3
https://microbiomejournal.biomedcentral.com/track/pdf/10.1186/s40168-021-01048-3
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