Comparison of Boiling and Robotics Automation Method in DNA Extraction for Metagenomic Sequencing of Human Oral Microbes

The rapid improvement of next-generation sequencing performance now enables us to analyze huge sample sets with more than ten thousand specimens. However, DNA extraction can still be a limiting step in such metagenomic approaches. In this study, we analyzed human oral microbes to compare the perform...

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Bibliographic Details
Main Authors: Junya Yamagishi, Yukuto Sato, Natsuko O. Shinozaki, Bin Ye, Akito Tsuboi, Masao Nagasaki, Riu Yamashita
Format: Artigo
Language:English
Published: 2016
Online Access:https://doi.org/10.1371/journal.pone.0154389
https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0154389&type=printable
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