Cloning and characterization of two cDNAs coding for human von Willebrand factor.
A cDNA library was prepared in lambda gt11 bacteriophage from poly(A)+ RNA isolated from primary cultures of endothelial cells from human umbilical vein. Approximately 2.5 million independent recombinants were screened and 2 of those were found to synthesize a fusion protein with beta-galactosidase...
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Principais autores: | , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
1985
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Acesso em linha: | https://doi.org/10.1073/pnas.82.19.6394 |
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