Development of a multiphoton fluorescence lifetime imaging microscopy system using a streak camera

We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (∼0.2 μm) and temporal (∼50 ps) resolution and allows rapid data acquisition and reliable and reproducible lifetime...

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Detalles Bibliográficos
Main Authors: R. V. Krishnan, H. Saitoh, Hirotoshi Terada, V. E. Centonze, Brian Herman
Formato: Artigo
Idioma:inglés
Publicado: 2003
Acceso en liña:https://doi.org/10.1063/1.1569410
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