Recombinant Human Cytochrome P450 1B1 Expression inEscherichia coli
Human cytochrome P450 (P450) 1B1 was expressed inEscherichia coliat a level of 200 nmol/liter culture using a pCW vector by removal of codons 2–4 and modification of the nucleotide sequence of the resulting N-terminal seven codons; a similar level of expression was found with a bicistronic construct...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Language: | English |
| Published: |
1998
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| Online Access: | https://doi.org/10.1006/abbi.1998.0808 |
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