Recombinant Human Cytochrome P450 1B1 Expression inEscherichia coli

Human cytochrome P450 (P450) 1B1 was expressed inEscherichia coliat a level of 200 nmol/liter culture using a pCW vector by removal of codons 2–4 and modification of the nucleotide sequence of the resulting N-terminal seven codons; a similar level of expression was found with a bicistronic construct...

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Bibliografske podrobnosti
Main Authors: Tsutomu Shimada, Rebecca M. Wunsch, Imad Hanna, Thomas R. Sutter, F.Peter Guengerich, Elizabeth M. J. Gillam
Format: Artigo
Jezik:angleščina
Izdano: 1998
Online dostop:https://doi.org/10.1006/abbi.1998.0808
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