High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA
ABSTRACT We describe a rapid and reproducible method for assessment of the hepatitis C virus (HCV) load in serum samples. The method combines Taqman technology (Roche) and the ABI Prism 7700 (Perkin Elmer) real-time sequence detection system. We have optimized a single-tube reverse transcription-PCR...
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Hoofdauteurs: | , , , , , , , |
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Formaat: | Artigo |
Taal: | Engels |
Gepubliceerd in: |
1999
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Online toegang: | https://doi.org/10.1128/jcm.37.2.327-332.1999 |
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