High-Throughput Real-Time Reverse Transcription-PCR Quantitation of Hepatitis C Virus RNA

ABSTRACT We describe a rapid and reproducible method for assessment of the hepatitis C virus (HCV) load in serum samples. The method combines Taqman technology (Roche) and the ABI Prism 7700 (Perkin Elmer) real-time sequence detection system. We have optimized a single-tube reverse transcription-PCR...

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Bibliografische gegevens
Hoofdauteurs: M. Martell, Jordi Gómez, Juan Ignacio Esteban, Sílvia Sauleda, Josep Quer, Beatriz Cabot, Rafael Esteban, Jaime Guardia
Formaat: Artigo
Taal:Engels
Gepubliceerd in: 1999
Online toegang:https://doi.org/10.1128/jcm.37.2.327-332.1999
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