Affinity purification of active plasminogen activator inhibitor-1 (PAI-1) using immobilized anhydrourokinase
Human Hep G2 hepatoma and HT 1080 fibrosarcoma cells were cultured in large scale under conditions which allowed enhanced secretion of plasminogen activator inhibitor-1 (PAI-1). A modified urokinase was obtained by reacting urokinase with phenylmethylsulfonyl fluoride followed by alkali treatment. T...
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| Glavni autori: | , , , |
|---|---|
| Format: | Artigo |
| Jezik: | engleski |
| Izdano: |
1989
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| Online pristup: | https://doi.org/10.1016/s0021-9258(18)83122-2 |
| Oznake: |
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