Elastin‐Like Polypeptides as a Purification Tag for Recombinant Proteins

Abstract This unit presents a recombinant protein purification method that employs an elastin‐like polypeptide (ELP) as a purification tag. ELPs undergo a sharp and reversible phase transition when heated above their lower critical solution temperature. ELPs retain this behavior when they are fused...

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Main Authors: Wafa Hassouneh, Trine Christensen, Ashutosh Chilkoti
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Acesso em linha:https://doi.org/10.1002/0471140864.ps0611s61
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