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Enhancing flavonoid production by systematically tuning the central metabolic pathways based on a CRISPR interference system in Escherichia coli -н Junjun Wu, Guocheng Du, Jian Chen, Jingwen Zhou

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Precise Gene Deletion and Replacement Using the CRISPR/Cas9 System in Human Cells -н Qiupeng Zheng, Xiaohong Cai, Meng How Tan, Steven Schaffert, Christopher P. Arnold, Xue Gong, Changzheng Chen, Shenglin Huang

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Regulation of the Type I-F CRISPR-Cas system by CRP-cAMP and GalM controls spacer acquisition and interference -н Adrian G Patterson, James T. Chang, Corinda Taylor, Peter C. Fineran

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The role of Cas8 in type I CRISPR interference -н Simon Cass, Karina A. Haas, Britta Stoll, Omer S. Alkhnbashi, Kundan Sharma, Henning Urlaub, Rolf Backofen, Anita Marchfelder, Edward L. Bolt

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<i>CFTR</i> Inactivation by Lentiviral Vector-mediated RNA Interference and CRISPR-Cas9 Genome Editing in Human Airway Epithelial Cells -н Jessica Bellec, Matthew Bacchetta, Davide Losa, Ignacio Anegón, Marc Chanson, Tuan M. Nguyen

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Sequence determinants of improved CRISPR sgRNA design -н Han Xu, Tengfei Xiao, Chen-Hao Chen, Wei Li, Clifford A. Meyer, Qiu Wu, Di Wu, Le Cong, Feng Zhang, Jun S. Liu, Myles Brown, X. Shirley Liu

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The New State of the Art: Cas9 for Gene Activation and Repression -н Marie La Russa, Lei S. Qi

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Clustered Regularly Interspaced Short Palindromic Repeat-Dependent, Biofilm-Specific Death of Pseudomonas aeruginosa Mediated by Increased Expression of Phage-Related Genes -н Gary E. Heussler, Kyle C. Cady, Katja Koeppen, Sabin Bhuju, Bruce A. Stanton, George A. O’Toole

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Next-generation libraries for robust RNA interference-based genome-wide screens -н Martin Kampmann, Max A. Horlbeck, Yuwen Chen, Jordan C. Tsai, Michael C. Bassik, Luke A. Gilbert, Jacqueline E. Villalta, S. Chul Kwon, Hyeshik Chang, V. Narry Kim, Jonathan S. Weissman

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Purification, crystallization, crystallographic analysis and phasing of the CRISPR-associated protein Csm2 from <i>Thermotoga maritima</i> -н Gloria Gallo, Gilles Augusto, Giulliana Rangel, André Zelanis, Marcelo A. Mori, Cláudia Barbosa Ladeira de Campos, Martin Würtele

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Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus -н Hirotaka Ebina, Naoko Misawa, Yuka Kanemura, Yoshio Koyanagi

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Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli -н Ido Yosef, Moran G. Goren, Udi Qimron

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Pervasive generation of oppositely oriented spacers during CRISPR adaptation -н Sergey Shmakov, Ekaterina Savitskaya, Ekaterina Semenova, Maria D. Logacheva, Kirill A. Datsenko, Konstantin Severinov

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Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3 -н Bei Gong, Minsang Shin, Jiali Sun, Che‐Hun Jung, Edward L. Bolt, John van der Oost, Jeong‐Sun Kim

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CRISPR Interference Can Prevent Natural Transformation and Virulence Acquisition during In Vivo Bacterial Infection -н David Bikard, Asma Hatoum-Aslan, Daniel Mucida, Luciano A. Marraffini

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Efficient genome editing in zebrafish using a CRISPR-Cas system -н Woong Y. Hwang, Yanfang Fu, Deepak Reyon, Morgan L. Maeder, Shengdar Q. Tsai, Jeffry D. Sander, Randall T. Peterson, Jing-Ruey Joanna Yeh, J. Keith Joung

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CRISPR adaptive immune systems of Archaea -н Gisle Vestergaard, Roger A. Garrett, Samir A. Shah

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Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9 -н Jinxiong Han, Jun Zhang, Li Chen, Bin Shen, Jiankui Zhou, Bian Hu, Yinan Du, Peri Tate, Xingxu Huang, Zhi‐Gang She

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Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems -н Akihiro Yasue, Silvia Naomi Mitsui, Takahito Watanabe, Tetsushi Sakuma, Seiichi Oyadomari, Takashi Yamamoto, Sumihare Noji, Taro Mito, Eiji Tanaka

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Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system -н Yicheng Zhao, Zhen Dai, Liang Huai Yang, Minghao Yin, Kuiying Ma, Mei He, Hongsheng Ouyang, Chun‐Bo Teng

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