Quantification of gallium cryo-FIB milling damage in biological lamellae

Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological processes in their native, cellular environment at atomic resolution. However, few cells are sufficiently thin to permit imaging with cryo-EM. Thinning of frozen cells to <500 nm lamellae by focused-ion-bea...

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Main Authors: Bronwyn A. Lucas, Nikolaus Grigorieff
格式: Artigo
語言:英语
出版: 2023
在線閱讀:https://doi.org/10.1073/pnas.2301852120
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