Quantification of gallium cryo-FIB milling damage in biological lamellae

Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological processes in their native, cellular environment at atomic resolution. However, few cells are sufficiently thin to permit imaging with cryo-EM. Thinning of frozen cells to <500 nm lamellae by focused-ion-bea...

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Main Authors: Bronwyn A. Lucas, Nikolaus Grigorieff
Formato: Artigo
Idioma:inglés
Publicado: 2023
Acceso en liña:https://doi.org/10.1073/pnas.2301852120
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