Quantification of gallium cryo-FIB milling damage in biological lamellae
Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological processes in their native, cellular environment at atomic resolution. However, few cells are sufficiently thin to permit imaging with cryo-EM. Thinning of frozen cells to <500 nm lamellae by focused-ion-bea...
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Main Authors: | Bronwyn A. Lucas, Nikolaus Grigorieff |
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Format: | Artigo |
Sprog: | engelsk |
Udgivet: |
2023
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Online adgang: | https://doi.org/10.1073/pnas.2301852120 |
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