Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM)

Abstract Visualization of RNAs in live cells is critical to understand biology of RNA dynamics and function in the complex cellular environment. Detection of RNAs with a fluorescent marker frequently involves genetically fusing an RNA aptamer tag to the RNA of interest, which binds to small molecule...

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Main Authors: Nadia Sarfraz, Emilia Moscoso, Therese Oertel, Harrison J. Lee, Suman Ranjit, Esther Braselmann
格式: Artigo
语言:英语
出版: 2023
在线阅读:https://doi.org/10.1038/s41467-023-36531-y
https://www.nature.com/articles/s41467-023-36531-y.pdf
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