Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM)
Abstract Visualization of RNAs in live cells is critical to understand biology of RNA dynamics and function in the complex cellular environment. Detection of RNAs with a fluorescent marker frequently involves genetically fusing an RNA aptamer tag to the RNA of interest, which binds to small molecule...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Language: | English |
| Published: |
2023
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| Online Access: | https://doi.org/10.1038/s41467-023-36531-y https://www.nature.com/articles/s41467-023-36531-y.pdf |
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