Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

Validation and standardization of DNA extraction and library construction methods for metagenomics-based human fecal microbiome measurements

Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research and its industrialization. This study set out to establish standards-based solutions to improve the accuracy and reproducibility of metag...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Dieter M. Tourlousse, Koji Narita, Takamasa Miura, Mitsuo Sakamoto, Akiko Ohashi, Keita Shiina, Masami Matsuda, Daisuke Miura, Mamiko Shimamura, Yoshifumi Ohyama, Atsushi Yamazoe, Y. Uchino, Keishi Kameyama, Shingo Arioka, Jiro Kataoka, Takayoshi Hisada, Kazuyuki Fujii, Shunsuke Takahashi, Miho Kuroiwa, Masatomo Rokushima, Mitsue Nishiyama, Yoshiki Tanaka, Takuya Fuchikami, Hitomi Aoki, Satoshi Kira, Ryo Koyanagi, Takeshi Naito, Morie Nishiwaki, Hirotaka Kumagai, Mikiko Konda, Ken Kasahara, Moriya Ohkuma, Hiroko Kawasaki, Yuji Sekiguchi, Jun Terauchi
Fformat: Artigo
Iaith:Saesneg
Cyhoeddwyd: 2021
Mynediad Ar-lein:https://doi.org/10.1186/s40168-021-01048-3
https://microbiomejournal.biomedcentral.com/track/pdf/10.1186/s40168-021-01048-3
Tagiau: Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!
Byddwch y cyntaf i adael sylw!
Rhaid i chi fewngofnodi yn gyntaf

Eitemau Tebyg