Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins

Significance Superresolution fluorescence microscopy (nanoscopy) has enabled the study of protein distributions in cellular organelles, albeit only down to a 3D resolution of 20 to 40 nm. By targeting single emitters with a doughnut-shaped excitation beam, the recently introduced MINFLUX nanoscopy p...

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Main Authors: Jasmin K. Pape, Till Stephan, Francisco Balzarotti, Rebecca Büchner, F. de Lange, Dietmar Riedel, Stefan Jakobs, Stefan W. Hell
格式: Artigo
語言:英语
出版: 2020
在線閱讀:https://doi.org/10.1073/pnas.2009364117
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