Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells
CRISPR (clustered, regularly interspaced, short palindromic repeats)/Cas9 (CRISPR-associated protein 9) has become the tool of choice for generating gene knockouts across a variety of species. The ability for efficient gene editing in primary T cells not only represents a valuable research tool to s...
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| Main Authors: | , |
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| 格式: | Artigo |
| 语言: | 英语 |
| 出版: |
2018
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| 在线阅读: | https://doi.org/10.1084/jem.20171626 |
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