Polyproline and the “spectroscopic ruler” revisited with single-molecule fluorescence

To determine whether Förster resonance energy transfer (FRET) measurements can provide quantitative distance information in single-molecule fluorescence experiments on polypeptides, we measured FRET efficiency distributions for donor and acceptor dyes attached to the ends of freely diffusing polypro...

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Main Authors: Benjamin Schuler, Everett A. Lipman, Peter Steinbach, Michael U. Kumke, William A. Eaton
Format: Artigo
Jezik:angleščina
Izdano: 2005
Online dostop:https://doi.org/10.1073/pnas.0408164102
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