Polyproline and the “spectroscopic ruler” revisited with single-molecule fluorescence
To determine whether Förster resonance energy transfer (FRET) measurements can provide quantitative distance information in single-molecule fluorescence experiments on polypeptides, we measured FRET efficiency distributions for donor and acceptor dyes attached to the ends of freely diffusing polypro...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Language: | English |
| Published: |
2005
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| Online Access: | https://doi.org/10.1073/pnas.0408164102 |
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