Polyproline and the “spectroscopic ruler” revisited with single-molecule fluorescence
To determine whether Förster resonance energy transfer (FRET) measurements can provide quantitative distance information in single-molecule fluorescence experiments on polypeptides, we measured FRET efficiency distributions for donor and acceptor dyes attached to the ends of freely diffusing polypro...
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Main Authors: | Benjamin Schuler, Everett A. Lipman, Peter Steinbach, Michael U. Kumke, William A. Eaton |
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格式: | Artigo |
语言: | 英语 |
出版: |
2005
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在线阅读: | https://doi.org/10.1073/pnas.0408164102 |
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