Use of aminoglycoside 3′ adenyltransferase as a selection marker for Chlamydia trachomatis intron-mutagenesis and in vivo intron stability
C hlamydia spp. are obligate, intracellular bacteria that infect humans and animals. Research on these important pathogens has been hindered due to a paucity of genetic tools. We recently adapted a group II intron (GII) mutagenesis platform for creation of ampicillin-selectable gene insertions in C....
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| Main Authors: | , , , , |
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| 格式: | Artigo |
| 语言: | 英语 |
| 出版: |
2015
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| 在线阅读: | https://doi.org/10.1186/s13104-015-1542-9 https://bmcresnotes.biomedcentral.com/counter/pdf/10.1186/s13104-015-1542-9 |
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