Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway
Abstract Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for production of therapeutic proteins. However, development of recombinant CHO cell lines has been hampered by unstable and variable transgene expression caused by random integration. Here we demonstrate efficient t...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | English |
| Published: |
2015
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| Online Access: | https://doi.org/10.1038/srep08572 https://www.nature.com/articles/srep08572.pdf |
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