Effect of flexibility and <i>cis</i> residues in single-molecule FRET studies of polyproline
Polyproline has recently been used as a spacer between donor and acceptor chromophores to help establish the accuracy of distances determined from single-molecule Förster resonance energy transfer (FRET) measurements. This work showed that the FRET efficiency in water is higher than expected for a r...
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| Main Authors: | , , , , , |
|---|---|
| 格式: | Artigo |
| 语言: | 英语 |
| 出版: |
2007
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| 在线阅读: | https://doi.org/10.1073/pnas.0709567104 |
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