Effect of flexibility and <i>cis</i> residues in single-molecule FRET studies of polyproline

Polyproline has recently been used as a spacer between donor and acceptor chromophores to help establish the accuracy of distances determined from single-molecule Förster resonance energy transfer (FRET) measurements. This work showed that the FRET efficiency in water is higher than expected for a r...

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Bibliographic Details
Main Authors: Robert B. Best, Kusai A. Merchant, Irina V. Gopich, Benjamin Schuler, Ad Bax, William A. Eaton
Format: Artigo
Language:English
Published: 2007
Online Access:https://doi.org/10.1073/pnas.0709567104
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