The T-cell-receptor repertoire in the synovial fluid of a patient with rheumatoid arthritis is polyclonal.
We have analyzed the T-cell-receptor repertoire expressed in the synovial fluid of a patient with rheumatoid arthritis by using an inverse polymerase chain reaction. Total RNA was isolated from Ficoll-purified mononuclear cells and converted into circularized double-stranded cDNA. Specific amplifica...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Language: | English |
| Published: |
1991
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| Online Access: | https://doi.org/10.1073/pnas.88.19.8534 |
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