Optimization of medium formulation and seed conditions for expression of mature PsaA (pneumococcal surface adhesin A) in Escherichia coli using a sequential experimental design strategy and response surface methodology - Ariane Leites Larentis; Júlia Fabiana Monteiro Quintal Nicolau; Ana Argondizzo; Ricardo Galler; Maria Isabel Rodrigues; Marco Alberto Medeiros (2012)