Optimal Temperature Selection for Mutation Detection by Denaturing HPLC and Comparison to Single-Stranded Conformation Polymorphism and Heteroduplex Analysis
Denaturing HPLC (DHPLC) is a semi-automated method for detecting unknown DNA sequence variants. The sensitivity of the method is dependent on the temperature at which the analysis is undertaken, the selection of which is dependent on operator experience. To circumvent this, software has been develop...
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| Main Authors: | , , , , , , |
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| 格式: | Artigo |
| 语言: | 英语 |
| 出版: |
1999
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| 在线阅读: | https://doi.org/10.1093/clinchem/45.8.1133 https://academic.oup.com/clinchem/article-pdf/45/8/1133/31929085/clinchem1133.pdf |
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