Optimal Temperature Selection for Mutation Detection by Denaturing HPLC and Comparison to Single-Stranded Conformation Polymorphism and Heteroduplex Analysis

Denaturing HPLC (DHPLC) is a semi-automated method for detecting unknown DNA sequence variants. The sensitivity of the method is dependent on the temperature at which the analysis is undertaken, the selection of which is dependent on operator experience. To circumvent this, software has been develop...

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主要な著者: Alistair C. Jones, Jehannine Austin, Nancy F. Hansen, Bastiaan Hoogendoorn, Peter J. Oefner, Jeremy P. Cheadle, Michael O’Donovan
フォーマット: Artigo
言語:英語
出版事項: 1999
オンライン・アクセス:https://doi.org/10.1093/clinchem/45.8.1133
https://academic.oup.com/clinchem/article-pdf/45/8/1133/31929085/clinchem1133.pdf
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