Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells
In order to clone novel diacylglycerol kinase (DGK) isozymes, we first obtained a DGK-related cDNA fragment by polymerase chain reaction using the human hepatoma cell line HepG2 mRNA and degenerated primers. The amplified fragment was subsequently used as a probe for screening the cDNA library from...
Shranjeno v:
Main Authors: | , , , , |
---|---|
Format: | Artigo |
Jezik: | angleščina |
Izdano: |
1994
|
Online dostop: | https://doi.org/10.1016/s0021-9258(17)32336-0 |
Oznake: |
Označite
Brez oznak, prvi označite!
|