Molecular cloning of a diacylglycerol kinase isozyme predominantly expressed in human retina with a truncated and inactive enzyme expression in most other human cells
In order to clone novel diacylglycerol kinase (DGK) isozymes, we first obtained a DGK-related cDNA fragment by polymerase chain reaction using the human hepatoma cell line HepG2 mRNA and degenerated primers. The amplified fragment was subsequently used as a probe for screening the cDNA library from...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Language: | English |
| Published: |
1994
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| Online Access: | https://doi.org/10.1016/s0021-9258(17)32336-0 |
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