Cation binding and conformational changes in VILIP and NCS-1, two neuron-specific calcium-binding proteins
VILIP and NCS-1, neural-specific, 22-kDa Ca2+-binding proteins possessing four EF-hands, were expressed in Escherichia coli to study their divalent cation properties.Flow dialysis (Ca2+ binding) and equilibrium gel filtration (Mg2' binding) revealed that both recombinant proteins possess only t...
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| Principais autores: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1994
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| Acesso em linha: | https://doi.org/10.1016/s0021-9258(20)30063-6 |
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